Delta Precision Ltd.
Expanded Bed Absorption chromatography (EBA) allows clarification and direct product capture in one unit operation.
Upflow conditions achieve a fluidised bed, twice the volume of the settled bed. Crude, high cell density and highly-viscous feed streams such as cell culture, foods and plasma are injected upflow. Cells, cell debris, host cell proteins (HCP's) and DNA leave the expanded bed, whilst the target molecule is bound to the media.
In order to facilitate a renaissance in EBA, columns built to current Good Manufacturing Practice (cGMP) standards are designed to be scalable and both feasible and economic to manufacture.
Laboratory columns at 10 and 20mm internal diameter, permitting small-scale process research, were modified to include an ultrasonic transceiver potted inside the piston, using published parameters for band width, etc. (Hofmann, 2003).